Journal: Frontiers in Cell and Developmental Biology
Article Title: The Glypican-1/HGF/C-Met and Glypican-1/VEGF/VEGFR2 Ternary Complexes Regulate Hair Follicle Angiogenesis
doi: 10.3389/fcell.2021.781172
Figure Lengend Snippet: Characterization of GPC1 expression in hair follicle sections and in KORS, HHFDPC and HDMEC cultures. (A) Representative GPC1 labeling (green) in hair follicle sections counterstained with Evans blue dye (red) of a 64-year-old donor. A schematic representation of a hair follicle is presented (adapted from (1)). (B–D) Cell morphology (10x), GPC gene expression analyzed by real time RT-PCR (mean ± SEM, n = 10) and GPC1 protein expression analyzed by Western immunoblotting of KORS, respectively. (E–G) Cell morphology (10x), GPCs gene expression analyzed by real-time RT-PCR (mean ± SEM, n = 10) and GPC1 protein expression analyzed by Western immunoblotting of HHFDPCs, respectively. (H–J) Cell morphology (10x), GPCs gene expression analyzed by real time RT-PCR (mean ± SEM, n = 10) and GPC1 protein expression analyzed by Western immunoblotting of HDMECs, respectively.
Article Snippet: Human hair follicle dermal papilla cells (HHFDPC) , PromoCell (403Z014.6) , Mesenchymal stem cell medium (MSCM) +5% FBS and growth factors.
Techniques: Expressing, Labeling, Quantitative RT-PCR, Western Blot